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Nanoscale diversity of synapses in the brain

Dive into our research focusing on the nano-organization of functionally distinct synapses within the brain. Through the application of Stimulated Emission Depletion (STED) super-resolution microscopy, we've delved into thalamocortical and cortico-cortical synapses in the somatosensory cortex, revealing notable insights.

 

Our observations indicate that glutamatergic synapses exhibit layer-specific nano-organization, suggesting a nuanced functional diversity across cortical layers. This implies the utilization of varied molecular programs to orchestrate pre- and post-synaptic architecture, supporting specific synaptic functions.

 

Our research is centered on understanding the mechanisms underlying this diverse molecular organization, with a particular focus on cell adhesion molecules like EphB RTK and ephrin-Bs. These molecules, known for their bi-directional signaling capabilities across the synaptic cleft, hold promise in establishing unique nanoscale compartments within synapses.

 

Join us in unraveling the complexities of synaptic function and molecular organization within the brain.

Confocal  3D image of thalamocortical and cortico-cortical synapses

STED  3D image of PSD-95 and Bassoon in TC and CC synapses

 3D reconstruction of TC and CC synapse nano-architecture

Nanoscale organization of thalamocortical synapses in the somatosensory cortex changes across cortical layers

​We are interested in understanding following questions:

  1. How are nano-domains established in diverse synaptic sub-types across distinct brain regions and layers?

  2. How are AMPAR and NMDAR organized in these synapses?

  3. What are the functional implications of diverse synaptic nano-organization for synaptic plasticity and cortical processing?

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